IB47230 Mini Genomic Plant DNA Sample Kit for 4 Preparations

The Genomic DNA mini kit (plant) provides a quick and easy method for purifying total DNA (including genomic DNA, mitochondrial and chloroplast DNA) from plant tissue. Samples are disrupted by both grinding in liquid nitrogen and lysis buffer incubation. The lysate is treated with RNase A to degrade RNA and then filtered to remove cell debris and salt precipitates. In the presence of the binding buffer, coupled with chaotropic salt, genomic DNA in the lysate binds to the glass fiber matrix of the spin column (1). Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer or TE. The procedure does not require DNA phenol extraction or alcohol precipitation, and can be completed in less than 1 hour. The purified genomic DNA is ready for use in PCR, Real-time PCR, Southern Blotting and RFLP. The quality of the Genomic DNA mini kit (plant) is tested on a lot-to-lot basis by isolating genomic DNA from 50 mg young leaf samples. More than 10 microgram of genomic DNA is quantified with a spectrophotometer and checked by electrophoresis. Caution: The components contain irritants. During operation, always wear a lab coat, disposable gloves, and protective goggles. Operation: centrifuge/vacuum. Binding capacity: 50 microgram. Expectant yield: up to 50 microgram of DNA. Operation time: 60 minutes or less.

• Kit contains 4 Mini GD columns, (8) 2mL collection tubes, (4) filter column, 2mL GP1 buffer, 2mL GPX1 buffer, 1mL GP2 buffer, 1.5mL GP3 buffer (3mL add isopropanol)
• It also contains 2mL W1 buffer, 1mL wash buffer (4mL add ethanol), 1mL elution buffer and 25 microliter RNASE A (10mg/mL)
• For research use only, extract genomic DNA from plant and fungal tissues
• Sample size: up to 100 mg of fresh or 25 mg of dry plant tissue
• Spin column format